Point of comparisonĪ procedure used to identify a specific sequence of DNA. Image 12: The image shows the basic comparison between southern blot, northern blot, and western blot. Used as a definite measure for Lyme disease, herpes, hepatitis B, and Creutzfeldt-Jacob disease.Detecting the presence of HIV antibody in serum.Detecting the presence of the virus and/or bacteria in the serum.Identifying the number of protein in a given sample.
Another term for western blot is protein blot or immune-blotting. It consists of different steps such as electrophoresis, transfer, and detection of specific proteins. Western blotting helps to detect a particular amino acid sequence in a mixture of protein. This procedure separates protein according to its size and in a straight column so that researchers can easily compare the amount of protein across various samples that run next to each other. Hybridization – It is a phenomenon in which a single-stranded DNA/RNA molecule anneals to complementary DNA/RNA.Denature – To alter or to take away the natural qualities, to destroy the characteristic properties using heat, acidity, and other measures.Capillary transfer – The ability of the liquid to flow in a narrow space.Electrophoresis – A method used in a laboratory setting to separate charged molecules such as DNA with relation to the molecule’s size.Let us take a look at the differences between the three: Terms commonly used in performing blotting procedures. There are various types of blotting methods: western blot, southern blot, and northern blot.
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